Coding
Si-tag1+li

Part:BBa_K1592013:Design

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-09-08)

LIP prepro + E. coli ribosomal protein L2 (1-60,GS linker,202-273aa) + YLcwp3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
    Illegal XhoI site found at 605
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 626


Design Notes

In order to replace the domains more conveniently, we respectivey added BamHI, SalI, NdeI between LIP2 prepro, E. coli ribosomal protein L2(1-60,linker,203-273aa), GS linker and YLcwp3.Also we added 6Xhis-tag between LIP2 prepro and E. coli ribosomal protein L2 (1-60,linker,203-273aa) to do verification of immunofluorescence.


Source

E. coli ribosomal protein L2 was synthesized by IDT, LIP2 prepro and YLcwp3 were cloning from the plasmid JMP62, and we fused this three sequences.

References